Pharmacologically Active Ingredients IV

Effect Of Aloe Lectin On Deoxyribonucleic Acid Synthesis In Baby Hamster Kidney Cells Yagi A; Machii K; Nishimura H; Shida T; Nishioka I Experientia 41(5):669-71 1985 May 15 A homogeneous glycoprotein (mol. wt 40,000) containing 34% carbohydrate was isolated from Aloe arborescens var. natalensis. At a concentration of 5 micrograms/ml, this glycoprotein was shown to stimulate deoxyribonucleic acid (DNA) synthesis in baby hamster kidney (BHK) cells and to have the properties of a lectin which reacts with sheep blood cells. The chemical and physical properties of the glycoprotein (Aloe lectin) are also discussed. Isolation Of S cDNA For A Phosphoenolpyruvate Carboxylase From A Monocot CAM-Plant, Aloe Arborescens: Structure & Its Gene Expression Honda H; Okamoto T; Shimada H Life Science Institute, Mitsui Toatsu Chemicals, Inc. Plant Cell Physiol 37(6):881-8 1996 Sep A phosphoenolpyruvate carboxylase (PEPCase) cDNA was isolated from Aloe arborescens, a monocot CAM plant. Northern analysis of the PEPCase transcript indicated that it is specifically expressed in green leaves, strongly suggesting its involvement in CAM photosynthesis. No diurnal change in expression level was evident. Western blot analysis also showed no alteration of the amount of the PEPCase protein. These results suggest that circadian rhythm in PEPCase activity may be regulated post-translationally. The representative cDNA clone contained an ORF encoding 964 amino acid residues. Deduced amino acid sequence of the Aloe PEPCase is highly conserved as compared with other PEPCases. The phosphorylation site which may be modified by PEPC-kinase was conserved. An evolutional map with known PEPCases suggested that CAM-type PEPCases were located between C4 and housekeeping PEPCases. Further Studies Of The Glucomannan From Aloe Vahombe(Liliaceae) II. Partial Hydrolyses & NMR 13C Studies Radjabi-Nassab F; Ramiliarison C; Monneret C; Vilkas E Biochimie 66(7-8):563-7 1984 Jul-Aug The polysaccharide from Aloe vahombe (lilaceae) was submitted to partial hydrolyses by sulfuric, oxalic and phosphoric acid. Some oligosaccharides were isolated and investigated by chemical and 13C NMR spectroscopic methods. Their structure was determined. The results prove unambiguously that in some oligosaccharides were isolated and investigated by chemical and 13C NMR spectroscopic methods. Their structure was determined. The results prove unambiguously that in the analyzed material the D-mannose is linked to the D-glucose by beta-1—4 linkages to form a carbohydrate heteropolymer. Bioactive Anthraquinone Glycosides From Picramnia Antidesma Spp. Fessonia Solis PN; Ravelo AG; Gonzalez AG; Gupta MP; Phillipson JD Department Of Pharamacognosy, School Of Pharmacy Phytochemistry 38(2):477-80 1995 Jan A bioactivity guided fractionation, using KB cells and brine shrimp assays, of the methanolic extract from the leaves of Picramnia antidesma yielded two known anthraquinones, Aloe-emodin and Aloe-emodin anthrone, and three new Aloe-emodin C-glycosides, named picramnioside A, picramnioside B and picramnioside C. Structures were established by spectroscopic methods (UV, IR, mass spectrometry, 1H and 13C and 2D NMR including COSY 45, HMQC, HMBC and ROESY). CD was used to establish the absolute configuration of the picramniosides.