Immunological Benefits III

Aloesin Up-Regulates Cyclin E/CDK2 Kinase Activity Via Inducing The Protein Levels Of Cyclin E, CDK2 & CDC25A In SK-HEP-1 Cells Lee KY; Park JH; Chung MH; Park YI; Kim KW; Lee YJ; Lee SK College Of Pharmacy, Seoul National University Biochem Mol Biol Int 41(2):285-92 1997 Feb In the present study, we show that aloesin, which is a low molecular weight ingredients present in Aloe vera, stimulates the proliferation of cultured human hepatoma SK-HEP-1 cells. The incorporation of [3H] thymidine into DNA in the cell cultures was significantly increased at a dose of 10 microM aloesin. The aloesin-induced DNA synthesis appears to require newly synthesized proteins because cycloheximide treatment blocked the DNA synthesis evoked by this compound. We then examined whether this compound increases the intracellular levels of cell cycle regulators by immunoblotting. The data showed that aloesin increased the levels of cyclin E, CDK2, and CDC25A in SK-HEP-1 cells. In addition, immuno-complex kinase assays showed that aloesin up-regulated the enzyme activity of cyclin E/CDK2 kinase in a dose-dependent manner. Collectively, these results suggest that aloesin stimulates the proliferation of SK-HEP-1 cells by inducing the intracellular levels of cyclin E/CDK2 kinase complex and CDC25A, which, together, result in the up-regulation of cyclin E-dependent kinase activity. Effect Of Chemotherapy Combined With The Use Of Tissue Preparations On Nonspecific Immunity In Patients With Pulmonary Tuberculosis Nersesian ON; Bogatyreva EV Probl Tuberk ISS 1, 1990, P28-31 General and local nonspecific immunity was studied in 143 new cases of pulmonary tuberculosis (71 and 72 persons, respectively). The results showed that combination of chemotherapy using desensitizing agents and tissue preparations according to V. P. Filatov (a suspension of placenta tissue and Aloe) had an immunomodulating effect. The efficacy of the combined chemotherapy amounted to 87 per cent with an account of the general immunity status. Immunostimulant Properties Of An Extract Isolated& Partially Purified From Aloe Vahombe Solar S; Zeller H; Rasolofonirina N; Coulanges P; Ralamboranto L; Andriatsimahavandy AA; Rakotovao LH; Le Deaut JY Arch Inst Pasteur Madagascar 47(1):9-39 1980 When the mice are given a hypodermic infection of unrefined Vahombe extract, the Aloe called Vahombe is a liliaceous plant growing in the South of Madagascar, they are protected against the infection caused by the Klebsiella, a pneumonia vector to man, giving rise to an experimental septicaemia in the mouse. Neither bactericide nor bacteriostatic activity has been detected yet about Aloe extract. The anti-infectious activity is proportional to the dose of extract injected, the protecting power is the greatest when the mice have been treated with Aloe, two or three days previously to the infection due to Klebsiella pneumoniae. We have determined the LD50 (Lethal dose 50) for the check batches (non-treated mice) and for the batches of protected mice. We were able to show that the previous injection developed the resistance to infection, multiplied from thirtyfold to a hundredfold. We have tackled the purification of the substance—made soluble after lyophilisation of the crude extract—by means of filtration with Sephadex G50. It would be the first time, for all we know, that a substance endowed with organism. At present we are proceeding with the purification of the active principle and contemplating trying the protective power upon virus infections as well as upon cancerous or parasitic ones. Immunostimulating Properties Of An Extract Isolated From Aloe Vahombe. 2. Protection In Mice By Fraction F1 Against Infections By Listeria Monocytogenes, Yersinia Pestis, Candida Albicans & Plasmodium Berghei Brossat JY; Ledeaut JY; Ralamboranto L; Rakotovao LH; Solar S; Gueguen A; Coulanges P Arch Inst Pasteur Madagascar 48(1):11-34 1981 A partially purified extract of leaves of Aloe vahombe, a plant endemic in the south of Madagascar, administered intravenously to mice, protects them against infection of bacteria (Listeria monocytogenes, Yersinia pestis), parasites (Plasmodium berghei) and fungus (Candida albicans). The protective fraction must be administered two days before inoculation of the pathogenic agent. These results significantly confirm those we obtained in earlier study on mice infection by Klebsiella pneumoniae. Currently we are testing the protective action of the purified extract on the experimental development of sarcomas, and we are in the process of analyzing the mode of action of this non specific immunostimulant.